rabbit polyclonal anti runx2  (Bioss)

Journal: JBMR Plus

Article Title: Proteomic analysis and effects on osteogenic differentiation of exosomes from patients with ossification of the spinal ligament

doi: 10.1093/jbmrpl/ziaf021

Figure Lengend Snippet: (A) Western blots revealing Runx2 and Wnt3a expression at wk 2 and 3 following culture in osteogenic differentiation medium using exosome-penetrating (1.2 μm) and exosome-non-penetrating (0.03 μm) filters. (B) Expression levels of Runx2 and Wnt3a of OLF-derived and non-OLF-derived (control) cells co-cultured with 0.03- and 1.2-μm filters. ( n = 3 each). ** p <.01.

Article Snippet: The membranes were washed twice in PBS solution containing 0.05% Tween 20, then blocked by a mixture of 5% skimmed milk in PBS for 1 h at room temperature, and finally incubated overnight with one of the following antibodies at 4 °C: rabbit anti-Runx2 polyclonal Ab (dilution, 1:250, Proteintech) and rabbit anti-Wnt3a polyclonal Ab (dilution, 1:250, Proteintech) to assess the osteogenic differentiation, and rabbit anti-COLIVA1 polyclonal Ab (dilution, 1:250, GeneTex) rabbit anti-FMNL3 polyclonal Ab (dilution, 1:250, Proteintech), rabbit anti-mTOR polyclonal Ab (dilution, 1:250, Proteintech), or rabbit anti-PIP4K2B polyclonal Ab (dilution, 1:250, Proteintech) to confirm the differential expression of candidate proteins.

Techniques: Western Blot, Expressing, Derivative Assay, Control, Cell Culture

Journal: Drug Design, Development and Therapy

Article Title: Catalpol Enhances Osteogenic Differentiation of Human Periodontal Stem Cells and Modulates Periodontal Tissue Remodeling in an Orthodontic Tooth Movement Rat Model

doi: 10.2147/DDDT.S482969

Figure Lengend Snippet: CAT promotes the expression of osteogenic differentiation markers in hPDLSCs. ( A ) The protein levels of COL1, ALP, and RUNX2 with CAT (0, 1, 10, 100 μM) after osteogenic induction for 14 days. ( B ) The protein levels of p-AKT and ER-α with CAT (0, 1, 10, 100 μM) after osteogenic induction for 14 days. ( C – E ) The mRNA levels of COL1, ALP, and RUNX2 with CAT (0, 1, 10, 100 μM) after osteogenic induction for 14 days (one-way ANOVA). Error bars stand for mean ± SD. **** P < 0.0001. ( F – J ) Relative quantification of COL1 ( F ), ALP ( G ), RUNX2 ( H ), p-AKT ( I ), and ER-α ( J ) protein levels with CAT (0, 1, 10, 100 μM) after osteogenic induction for 14 days (one-way ANOVA). Error bars stand for mean ± SD. *** P < 0.001, **** P < 0.0001. Each experiment was repeated five times.

Article Snippet: Immerse the slide in a solution containing specific primary antibodies such as polyclonal anti-rabbit RUNX2 (1:200; ImmunoWay, Plano, TX, USA), COL-1 (1:300; Proteintech, Hubei, China), and RANKL (1:300; Solarbio, Beijing, China) antibodies and incubate overnight at 4°C.

Techniques: Expressing, Quantitative Proteomics

Journal: Drug Design, Development and Therapy

Article Title: Catalpol Enhances Osteogenic Differentiation of Human Periodontal Stem Cells and Modulates Periodontal Tissue Remodeling in an Orthodontic Tooth Movement Rat Model

doi: 10.2147/DDDT.S482969

Figure Lengend Snippet: CAT promoted osteogenic differentiation of hPDLSCs through the PI3K/AKT pathway. LY294002 successfully reversed the expression of osteogenic differentiation markers by CAT. ( A and B ) The protein levels of COL1, ALP, RUNX2, p-AKT, and ER-α following osteogenic induction for 14 days of the control group and CAT group (10 μM) with or without LY294002 added. ( C – G ) Relative quantification of COL1 ( C ), ALP ( D ), RUNX2 ( E ), p-AKT ( F ), and ER-α ( G ) protein levels following osteogenic induction for 14 days of the control group and CAT group (10 μM) with or without LY294002 added (one-way ANOVA). Error bars stand for mean ± SD. ** P < 0.01, *** P < 0.001, **** P < 0.0001. Each experiment was repeated five times.

Article Snippet: Immerse the slide in a solution containing specific primary antibodies such as polyclonal anti-rabbit RUNX2 (1:200; ImmunoWay, Plano, TX, USA), COL-1 (1:300; Proteintech, Hubei, China), and RANKL (1:300; Solarbio, Beijing, China) antibodies and incubate overnight at 4°C.

Techniques: Expressing, Control, Quantitative Proteomics

Journal: Drug Design, Development and Therapy

Article Title: Catalpol Enhances Osteogenic Differentiation of Human Periodontal Stem Cells and Modulates Periodontal Tissue Remodeling in an Orthodontic Tooth Movement Rat Model

doi: 10.2147/DDDT.S482969

Figure Lengend Snippet: CAT activated the PI3K/AKT pathway mediated by ER-α to promote the osteogenic differentiation of hPDLSCs. MPP successfully reversed the expression of osteogenic differentiation and pathway-related markers by CAT. ( A and B ) The protein levels of COL1, ALP, RUNX2, p-AKT, and ER-α following osteogenic induction for 14 days of the control group and CAT group (10 μM) with or without MPP added. ( C – G ) Relative quantification of COL1 ( C ), ALP ( D ), RUNX2 ( E ), p-AKT ( F ), and ER-α ( G ) protein levels following osteogenic induction for 14 days of the control group and CAT group (10 μM) with or without MPP added (one-way ANOVA). Error bars stand for mean ± SD. ** P < 0.01, *** P < 0.001, **** P < 0.0001. Each experiment was repeated five times.

Article Snippet: Immerse the slide in a solution containing specific primary antibodies such as polyclonal anti-rabbit RUNX2 (1:200; ImmunoWay, Plano, TX, USA), COL-1 (1:300; Proteintech, Hubei, China), and RANKL (1:300; Solarbio, Beijing, China) antibodies and incubate overnight at 4°C.

Techniques: Expressing, Control, Quantitative Proteomics